生物炭對土壤微生物量及其群落結構的影響Impacts of Biochar on Soil Microbial Biomass and Community Structure
蓋霞普;翟麗梅;王洪媛;劉宏斌;任天志;雷秋良;武淑霞;
摘要(Abstract):
大量研究表明,生物炭對不同類型土壤中微生物量及群落結構特征影響不同,為探討這種影響差異是來自生物炭類型還是土壤本身特征,選擇紅壤和黑土兩類典型土壤同時開展了生物炭對不同類型土壤微生物量及其群落結構的影響作用研究,為生物炭在不同類型農田土壤中的應用提供一定借鑒和參考。采用玉米秸稈生物炭(炭化溫度500℃)作為供試材料,分別按照炭土質量比0、1%、2%和4%施用于紅壤和黑土中,通過41d的好氣培養試驗,研究生物炭對土壤pH、有機碳(SOC)、全氮(TN)、銨態氮(NH_4~+-N)、硝態氮(NO_3~--N)、微生物量碳(MBC)、微生物量氮(MBN)的影響,以及磷脂脂肪酸(PLFA)表征的微生物群落結構的影響。結果表明:從時間尺度變化規律來看,兩類土壤中SOC和TN含量隨著培養時間的延長而逐漸降低,土壤NH_4~+-N、NO_3~--N、MBC以及MBN呈現波動性變化規律,這與土壤中可利用態碳氮養分消耗有關。從生物炭添加效果來看,生物炭對兩類土壤生化性質和微生物群落結構的影響差異顯著:生物炭能夠顯著提高紅壤pH,達1.9%~17.0%,而對黑土pH影響不明顯;生物炭能顯著提高紅壤和黑土中SOC含量,對紅壤SOC的提升潛力更大,達18.6%~85.7%,而對NH_4~+-N和NO_3~--N含量影響不顯著。生物炭會降低紅壤中MBC、MBN和PLFA含量及其微生物群落結構多樣性,降低幅度分別為17.4%~50.2%、19.8%~23.9%、24.9%~43.1%,對細菌/真菌沒有顯著影響;然而,生物炭的添加能夠提高黑土MBC、MBN和PLFA含量及其微生物群落結構多樣性,PLFA總量是對照的1.2~1.6倍,并增加微生物群落結構中細菌的比例。施用生物炭能夠改善紅壤和黑土的養分狀況,且對有機質含量較低的酸性紅壤的提升潛力更強;生物炭對土壤微生物量及群落結構的短期影響受特定土壤生化性質影響很大,其長期作用影響還需進一步研究。
關鍵詞(KeyWords): 生物炭;土壤養分;微生物量;磷脂脂肪酸;紅壤;黑土
基金項目(Foundation): 公益性行業(農業)科研專項經費項目(201303095);; 中央級公益性科研院所基金項目(1610132016055)
作者(Author): 蓋霞普;翟麗梅;王洪媛;劉宏斌;任天志;雷秋良;武淑霞;
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參考文獻(References):
- [1]LEHMANN J,JOSEPH S.Biochar for environmental management:an introduction.In:Lehmann J.and Joseph S.(Eds.),Biochar for environmental management-science and technology[M].Earthscan,UK and USA,2009:1-9.
- [2]KAMEYAMA K,MIYAMOTO T,SHIONO T,et al.Influence of sugarcane bagasse derived biochar application on nitrate leaching in calcaric dark red soil[J].Environmental Science&Technology,2012,41(4):1131-1137.
- [3]WANG X,ZHOU W,LIANG G,et al.Characteristi cs of maize biochar with different pyrolysis temperatures and its effects on organic carbon,nitrogen and enzymatic activities after addition to fluvo-aquic soil[J].Science of the Total Environment,2015,538:137-144.
- [4]王月玲,耿增超,尚杰,等.施用生物炭后塿土土壤有機碳、氮及碳庫管理指數的變化[J].農業環境科學學報,2016,35(3):532-539.
- [5]ATKINSON C J,FITZGERALD J D,HIPPS N A.Potential mechanisms for achieving agricultural benefits from biochar application to temperate soils:a review[J].Plant and Soil,2010,337(1-2),1-18.
- [6]ZHANG Q Z,DIJKSTRA F A,LIU X R,et al.Effects of biochar on soil microbial biomass after four years of consecutive application in the north China plain[J].PLo S ONE,2014,9(7):e102062.
- [7]張星,劉杏認,張晴雯,等.生物炭和秸稈還田對華北農田玉米生育期土壤微生物量的影響[J].農業環境科學學報,2015,34(10):1943-1950.
- [8]XU H J,WANG X H,LI H,et al.Biochar impacts soil microbial community composition and nitrogen cycling in an acidic soil planted with rape[J].Environmental Science&Technology,2014,48(16):9391-9399.
- [9]NELISSEN V,RUTTING T,HUYGENS D,et al.Temporal evolution of biochar’s impact on soil nitrogen processes under field conditions-a 15N tracing study[J].Global Change Biology Bioenergy,2015,7(4):635-645.
- [10]CROSS A,SOHI S P.The priming potential of biochar products in relation to labile carbon contents and soil organic matter status[J].Soil Biology&Biochemistry,2011,43(10):2127-2134.
- [11]李明,李忠佩,劉明,等.不同秸稈生物炭對紅壤性水稻土養分及微生物群落結構的影響[J].中國農業科學,2015,48(7):1361-1369.
- [12]姚玲丹,程廣煥,王麗曉,等.施用生物炭對土壤微生物的影響[J].環境化學,2015,34(4):697-704.
- [13]蓋霞普,劉宏斌,翟麗梅,等.玉米秸稈生物炭對中性水稻土養分和微生物群落結構影響的時間尺度變化研究[J].農業環境科學學報,2016,35(4):719-728.
- [14]XU N,TAN G C,WANG H Y,et al.Effect of biochar additions to soil on nitrogen leaching,microbial biomass and bacterial community structure[J].European Journal of Soil Biology,2016,74:1-8.
- [15]CHEN J H,LIU X Y,ZHENG J W,et al.Biochar soil amendment increased bacterial but decreased fungal gene abundance with shifts in community structure in a slightly acid rice paddy from Southwest China[J].Applied Soil Ecology,2013,71:33-44.
- [16]DEMPSTER D N,GLEESON D B,SOLAIMAN Z M,et al.Decreased soil microbial biomass and nitrogen mineralisation with Eucalyptus biochar addition to a coarse textured soil[J].Plant and Soil,2012,354(1-2):311-324.
- [17]王洪媛,蓋霞普,翟麗梅,等.生物炭對土壤氮循環的影響研究進展[J].生態學報,2016,36(19):5998-6011.
- [18]蓋霞普,劉宏斌,翟麗梅,等.玉米秸稈生物炭對土壤無機氮素淋失風險的影響研究[J].農業環境科學學報,2015,34(2):310-318.
- [19]DING Y,LIU Y X,WU W X,et al.Evaluation of biochar effects on nitrogen retention and leaching in multi-layered soil columns[J].Water Air and Soil Pollution,2010,213(1-4):47-55.
- [20]魯如坤.土壤和農業化學分析法[M].北京:中國農業科技出版社,2000.
- [21]FROSTEG A觷RD A,TUNLID A,B A觷A觷TH F.Phospholipid fatty acid composition,biomass and activity of microbial communities from two soil types experimentally exposed to different heavy metals[J].Applied Environmental Microbiology,1993,59(11):3605-3617.
- [22]VAN ZWIETEN L,KIMBER S,MORRIS S,et al.Effects of biochar from slow pyrolysis of papermill waste on agronomic performance and soil fertility[J].Plant and Soil,2010,327:235-246.
- [23]王曙光,候彥林.磷酸脂肪酸的方法在土壤微生物分析中的應用[J].微生物學通報,2004,31:114-117.
- [24]黃超,劉麗君,章明奎.生物質炭對紅壤性質和黑麥草生長的影響[J].浙江大學學報:農業與生命科學版,2011,37(4):439-445.
- [25]STEWART C,ZHENG J,BOTTE J,et al.Co-generated fast pyrolysis biochar mitigates green-house gas emissions and increases carbon sequestration in temperate soils[J].Global Change Biology Bioenergy,2013,5(Suppl.2):153-164.
- [26]SCHMIDT M W I,NOACK A G.Black carbon in soils and sediments:Analysis,distribution,implications,and current challenges[J].Global Biogeochemical Cycles,2000,14(3):777-794.
- [27]高海英,何緒生,陳心想,等.生物炭及炭基硝酸銨肥料對土壤化學性質及作物產量的影響[J].農業環境科學學報,2012,31(10):1948-1955.
- [28]LEHMANN J,GAUNT J,RONDON M.Biochar sequestration in terrestrial ecosystems-a review[J].Mitigation and Adaptation Strategies for Global Change,2006,11:395-419.
- [29]MUKHERJEE A,ZIMMERMAN A R.Organic carbon and nutrient release from a range of laboratory-produced biochars and biochar-soil mixtures[J].Geoderma,2013,193-194:122-130.
- [30]JU X,XING G,CHEN X,et al.Reducing environmental risk by improving N management in intensive Chinese agricultural systems[J].Proceedings of the National Academy of Science,2009,106,3041-3046.
- [31]ACIEGO PIETRI J C,BROOKES P C.Relationships between soil p H and microbial properties in a UK arable soil[J].Soil Biology&Biochemistry,2008,40(7):1856-1861.
- [32]吳超,曲東,劉浩.初始p H值對堿性和酸性水稻土微生物鐵還原過程的影響[J].生態學報,2014,34(4):933-942.
- [33]BERGLUND L M,DELUCA T H,ZACKRISSON O.Activated carbon amendments to soil alters nitrification rates in Scots pine forests[J].Soil Biology&Biochemistry,2004,36(12):2067-2073.
- [34]AMELOOT N,NEVE S D,JEGAJEEVAGAN K,et al.Short-term CO2and Nx O emissions and microbial properties of biochar amended sandy loam soils[J].Soil Biology&Biochemistry,2013,57,401-410.
- [35]LUO Y,DURENKAMP M,NOBILI M D,et al.Microbial biomass growth following incorporation of biochars produced at 350℃or 700℃,in a silty-clay loam soil of high and low p H[J].Soil Biology&Biochemistry,2013,57,513-523.
- [36]LEHMANN J,RILLIG M C,THIES J,et al.Biochar effects on soil biota-A review[J].Soil Biology&Biochemistry,2011,43(9):1812-1836.
- [37]陳偉,周波,束懷瑞.生物炭和有機肥處理對平邑甜茶根系和土壤微生物群落功能多樣性的影響[J].中國農業科學,2013,46(18):3850-3856.
- [38]NELISSEN V,RUYSSCHAET G,MANKA’ABUSI D,et al.Impact of a woody biochar on properties of a sandy loam soil and spring barley during a two-year field experiment[J].European Journal of Agronomy,2015,62:65-78.
- [39]SUN D,JUN M,ZHANG W,et al.Implication of temporal dynamics of microbial abundance and nutrients to soil fertility under biochar application-field experiments conducted in a brown soil cultivated with soybean,north China[J].In:SUN D,JUN M,ZHANG W et al.(Eds.),Advances in Environmental Science and Engineering,518-523.Trans Tech Publications,Switzerland,2012:384-394.
- [40]GOMEZ J D,DENEF K,STEWART C E,et al.Biochar addition rate influences soil microbial abundance and activity in temperate soils[J].European Journal of Soil Science,2014,65(1):28-39.
- [41]FARRELL M,KUHN T K,MACDNALD L M,et al.Microbial utilization of biochar-derived carbon[J].Science of the Total Environment,2013,465:288-297.
- [42]FARRELL M,KUHN T K,MACDONALD L M,et al.Microbial utilization of biochar-derived carbon[J].Science of the Total Environment,2013,465(SI):288-297.
- [43]MUHAMMAD N,DAI Z,XIAO K,et al.Changes in microbial community structure due to biochars generated from different feedstocks and their relationships with soil chemical properties[J].Geoderma,2014,226:270-278.
- [44]BAMMINGER C,ZAISER N,ZINSSER P,et al.Effects of biochar,earthworms,and litter addition on soil microbial activity and abundance in a temperate agricultural soil[J].Biology and Fertility of Soils,2014,50(8):1189-1200.
- [45]JINDO K,SNCHEEZ-MONEDERO M A,Hernández T,et al.Biochar influences the microbial community structure during manure composting with agricultural wastes[J].Science of the Total Environment,2012,416:476-481.